Product Info Summary
SKU: | PA1320 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-PPAR gamma/PPARG Antibody
View all PPAR gamma/NR1C3 Antibodies
SKU/Catalog Number
PA1320
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPAR gamma/PPARG Antibody catalog # PA1320. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPAR gamma/PPARG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1320)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma, different from the rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1320 is reactive to PPARG in Human
Applications
PA1320 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
57.62kDa
Background of PPAR gamma/NR1C3
The peroxisome proliferator-activated receptors (PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al. (2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-PPAR gamma antibody, PA1320, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HT1080 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For PPARG (Source: Uniprot.org, NCBI)
Gene Name
PPARG
Full Name
Peroxisome proliferator-activated receptor gamma
Weight
57.62kDa
Superfamily
nuclear hormone receptor family
Alternative Names
CIMT1; NR1C3; NR1C3GLM1; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma; PPAR gamma; PPARG; PPARG1peroxisome proliferative activated receptor, gamma; PPARG2PPARgamma; PPAR-gamma PPARG CIMT1, GLM1, NR1C31, PPARG2, PPARG5, PPARgamma, PPARG peroxisome proliferator activated receptor gamma peroxisome proliferator-activated receptor gamma|PPAR-gamma|nuclear receptor subfamily 1 group C member 3|peroxisome proliferator-activated nuclear receptor gamma variant 1|peroxisome proliferator-activated receptor-gamma 5|peroxisome proliferator-activated receptor-gamma splicing
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPARG, check out the PPARG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPARG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPAR gamma/PPARG Antibody (PA1320)
Hello CJ!
PA1320 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births
Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
Species: Human
PA1320 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
Rosiglitazone infusion therapy following minimally invasive surgery for intracerebral hemorrhage evacuation decreases matrix metalloproteinase-9 and blood-brain barrier disruption in rabbits.
Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice
Electrospun Poly(L-lactide)/Poly(?-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells
Scorpion in Combination with Gypsum: Novel Antidiabetic Activities in Streptozotocin-Induced Diabetic Mice by Up-Regulating Pancreatic PPAR
Wang W, Liu L, Dang X, Ma S, Zhang M, Wang K. Bmc Musculoskelet Disord. 2012 Aug 9;13:142. Doi: 10.1186/1471-2474-13-142. The Effect Of Core Decompression On Local Expression Of Bmp-2, Ppar-?? And Bone Regeneration In The Steroid-Induced Femoral H...
Jiang Y, Zhang Y, Zhang H, Zhu B, Li P, Lu C, Xu Y, Chen W, Lin N. Exp Biol Med (Maywood). 2014 Mar;239(3):347-55. Doi: 10.1177/1535370213519215. Epub 2014 Feb 7. Pravastatin Prevents Steroid-Induced Osteonecrosis In Rats By Suppressing Ppar?? Exp...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-PPAR gamma/PPARG Antibody
Question
Would PA1320 anti-PPAR gamma/PPARG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
You can see on the product datasheet, PA1320 anti-PPAR gamma/PPARG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-23
Question
Does anti-PPAR gamma/PPARG antibody PA1320 work for WB with subcutaneous adipose tissue?
W. Walker
Verified customer
Asked: 2020-04-15
Answer
According to the expression profile of subcutaneous adipose tissue, PPARG is highly expressed in subcutaneous adipose tissue. So, it is likely that anti-PPAR gamma/PPARG antibody PA1320 will work for WB with subcutaneous adipose tissue.
Boster Scientific Support
Answered: 2020-04-15
Question
Is this PA1320 anti-PPAR gamma/PPARG antibody reactive to the isotypes of PPARG?
D. Thomas
Verified customer
Asked: 2020-04-03
Answer
The immunogen of PA1320 anti-PPAR gamma/PPARG antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma(45-62aa PHYEDIPFTRTDPVVADY), different from the rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-03
Question
I see that the anti-PPAR gamma/PPARG antibody PA1320 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-19
Question
We are currently using anti-PPAR gamma/PPARG antibody PA1320 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-PPAR gamma/PPARG antibody (PA1320) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
I was wanting to use your anti-PPAR gamma/PPARG antibody for WB for human subcutaneous adipose tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human subcutaneous adipose tissue identification?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
As indicated on the product datasheet, PA1320 anti-PPAR gamma/PPARG antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human subcutaneous adipose tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
We have seen staining in human placenta. Are there any suggestions? Is anti-PPAR gamma/PPARG antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
From literature placenta does express PPARG. From Uniprot.org, PPARG is expressed in subcutaneous adipose tissue, heart, adipose tissue, bone marrow, placenta, cervix carcinoma, macrophage, among other tissues. Regarding which tissues have PPARG expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8702406, 9144532
Bone marrow, Pubmed ID: 7787419
Cervix carcinoma, Pubmed ID: 23186163
Heart, Pubmed ID: 9065481
Macrophage, Pubmed ID: 25504154
Placenta, Pubmed ID: 8706692, 9356045, 15489334
Boster Scientific Support
Answered: 2019-09-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for subcutaneous adipose tissue using anti-PPAR gamma/PPARG antibody PA1320. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
Does anti-PPAR gamma/PPARG antibody PA1320 work on feline WB with bone marrow?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Our lab technicians have not tested anti-PPAR gamma/PPARG antibody PA1320 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PPAR gamma/PPARG antibody PA1320 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline bone marrow in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-29
Question
Is a blocking peptide available for product anti-PPAR gamma/PPARG antibody (PA1320)?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
We do provide the blocking peptide for product anti-PPAR gamma/PPARG antibody (PA1320). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-12
Question
I am interested in to test anti-PPAR gamma/PPARG antibody PA1320 on human subcutaneous adipose tissue for research purposes, then I may be interested in using anti-PPAR gamma/PPARG antibody PA1320 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
The products we sell, including anti-PPAR gamma/PPARG antibody PA1320, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-01
Question
My question regards using your anti-PPAR gamma/PPARG antibody for activation of cysteine-type endopeptidase activity involved in apoptotic process studies. Has this antibody been tested with western blotting on mm231 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
Thank you for your inquiry. This PA1320 anti-PPAR gamma/PPARG antibody is tested on mm453 cell lysate, mm231 cell lysate, hela cell lysate, jurkat cell lysate, ht1080 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-18
Question
Do you have a BSA free version of anti-PPAR gamma/PPARG antibody PA1320 available?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PPAR gamma/PPARG antibody PA1320 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-13
Question
My team were satisfied with the WB result of your anti-PPAR gamma/PPARG antibody. However we have seen positive staining in subcutaneous adipose tissue nucleus. cytoplasm. note=redistributed from using this antibody. Is that expected? Could you tell me where is PPARG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
Based on literature, subcutaneous adipose tissue does express PPARG. Generally PPARG expresses in nucleus. cytoplasm. note=redistributed from. Regarding which tissues have PPARG expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8702406, 9144532
Bone marrow, Pubmed ID: 7787419
Cervix carcinoma, Pubmed ID: 23186163
Heart, Pubmed ID: 9065481
Macrophage, Pubmed ID: 25504154
Placenta, Pubmed ID: 8706692, 9356045, 15489334
Boster Scientific Support
Answered: 2018-01-10
Question
Can you help my question with product PA1320, anti-PPAR gamma/PPARG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1320 anti-PPAR gamma/PPARG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-29
Question
Here is the WB image, lot number and protocol we used for subcutaneous adipose tissue using anti-PPAR gamma/PPARG antibody PA1320. Please let me know if you require anything else.
J. Parker
Verified customer
Asked: 2017-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-10